The genus Bifidobacteriaceae consists of Gram-positive, rod-shaped bacteria with a characteristic bifid (branching) appearance at their ends, which is why they are named "Bifidobacteriaceae." These bacteria are strict anaerobes and are widely found in the digestive tracts, vaginas, and oral cavities of humans and animals. The colony morphology, appearance, and size of Bifidobacteriaceae can vary depending on the strain characteristics and nutritional conditions. Bifidobacteriaceae species are crucial members of the gut microbiota and play a key role in maintaining gut heath and overall well-being. As probiotics, they not only support the balance of the qut microbiome but also interact beneficially with the host's immune and digestive systems. These characteristics make Bifidobacteriaceae a focal point in health and nutrition research and applications.