Lactobacillus Acidophilus LA85-a good helper in preventing diarrhea

Midsummer is approaching, the "summer" eating pattern has begun, and many pathogenic microorganisms are also multiplying rapidly.

Raw and cold diets significantly increase the risk of intestinal diseases, and diarrhea becomes the most common intestinal disease in summer.

Causes of diarrhea:

Diarrhea is a group of intestinal diseases caused by multiple pathogens and factors. In mild cases, it may be accompanied by vomiting. In severe cases, it may lead to varying degrees of dehydration, acidosis and electrolyte imbalance. In severe cases, it may even be life-threatening. Why does diarrhea occur?

Diarrheal diseases are mainly caused by pathogenic microorganisms such as viruses and bacteria, and bacterial diarrhea is more common in daily diet and life, mainly caused by Salmonella, enterotoxigenic Escherichia coli(ETEC), Vibrio cholerae, and Campylobacter.

Antibiotic-associated diarrhea is a common intestinal flora imbalance disease caused by the overuse of antibiotics. It kills antibiotic-sensitive strains while allowing resistant strains to not only survive but multiply. Antibiotics interfere with bacterial metabolism and destroy the balance of intestinal flora.

Probiotics and antibiotics are commonly used treatment options for people with diarrhea. However, the abuse of antibiotics is another cause of frequent diarrhea. People are looking forward to more excellent probiotic diarrhea treatments.

In order to meet the needs of consumers, Wecare Probiotics has demonstrated its ability to select high-quality strains and launched a related patented probiotic strain - Lactobacillus acidophilus LA85. Patent name: A Lactobacillus acidophilus used in the preparation of prevention or application in food or medicine to treat antibiotic-associated diarrhea, patent number pending: CN202210349140.4.

Antagonistic inhibition, source solution

Inhibit pathogenic bacteria and prevent diarrhea

● Lactobacillus acidophilus LA85 effectively inhibits intestinal pathogenic bacteria

In order to study the antagonistic effect of Lactobacillus acidophilus LA85 on human pathogenic bacteria that cause diarrhea, Wecare Probiotics conducted relevant experiments. According to the research results, Lactobacillus acidophilus LA85 has an inhibitory effect on most harmful intestinal bacteria. The opportunistic pathogenic bacteria Escherichia coli, Salmonella and Staphylococcus aureus have obvious inhibitory effects.

Inhibitory effect of Lactobacillus acidophilus LA85 fermentation broth on pathogenic bacteria

It can be seen from the above table that when the indicator bacteria concentration is 106 CFU/mL, Lactobacillus acidophilus LA85 has a very strong inhibitory effect on the growth of Staphylococcus aureus, Escherichia coli and Salmonella, especially the strongest inhibitory effect on Escherichia coli. The diameter of the inhibition zone reaches 44mm. When the concentration of the indicator bacteria was adjusted to 108 CFU/mL, Lactobacillus acidophilus LA85 still showed a strong inhibitory effect on the three pathogenic bacteria.

● Lactobacillus acidophilus LA85 prevents or treats antibiotic-associated diarrhea (ADD)

For the associated diarrhea caused by antibiotic drugs, Lactobacillus acidophilus LA85 also significantly alleviated antibiotic-associated diarrhea and significantly improved the lesions of antibiotic-associated diarrhea.

Research shows that products with LA85 strain as the main functional ingredient can be specifically expressed in:

1. Significantly increased the body weight of mice with antibiotic-associated diarrhea;

2. Significantly reduced disease activity index in mice with antibiotic-associated diarrhea;

3. Increase the diversity of intestinal lactase in ADD mice;

4. Significantly increased SOD and GSH levels and reduced MDA levels in ADD mice;

5. Reduce the levels of serum pro-inflammatory cell inflammatory factors: TNF-ɑ, IL-1b and IL-6 in mice with antibiotic-associated diarrhea.

Therefore, there is good prospect for its use in the preparation of products for the prevention and/or treatment of antibiotic-associated diarrhea or antibiotic-associated diarrhea.

Major strains with high activity, high stability, strong colonization

● The highly active strain LA85 is resistant to gastric acid and bile salts

According to experiments, Lactobacillus acidophilus powder LA85 acts for 2 hours under simulated gastric acid pH 3.0 conditions, and the survival rate reaches 80%. After 2 hours of simulated bile salt concentration of 0.3%, the survival rate reached 85%. 

● Highly active strain LA85 features strong adhesion properties, strong intestinal colonization ability, and long shelf life

To further examine the adhesion properties of Lactobacillus acidophilus LA85, Caco-2 cells were selected as the research object. The results are shown in Figure 1. LA85 has good adhesion ability to Caco-2 cells, and the number of bacteria adhered to each cell exceeds 15, higher than the control strain LA-05.

Intestinal epithelial cell adhesion properties of strains

At the same time, in terms of intestinal colonization performance, according to experimental data (Figure 2), Lactobacillus acidophilus strain LA85 has good colonization ability in the intestine.

Colonization ability of Lactobacillus acidophilus LA85 in mouse intestine

Data source: Invention patent 1. A kind of Lactobacillus acidophilus with probiotic properties and its application (ZL202110285880.1) 2. A kind of Lactobacillus acidophilus in the preparation of food or medicine for preventing or treating antibiotic-associated diarrhea application (CN202210349140.4 is under application)

Exploring high-quality bacteria in various aspects to help quality life

Wecare Probiotics Diarrhea Probiotic Solution

As a leading supplier of raw materials for probiotic strains, Wecare Probiotics launches probiotic solutions for diarrhea, using superior and patented strains, and scientific compounding to form highly active and stable formulas to help customers create health products that are more suitable for consumers.

Lactobacillus plantarum Lp90 related patents:

1. A kind of Lactobacillus acidophilus with probiotic properties and its application (ZL202110285880.1)

2. Application of Lactobacillus acidophilus in the preparation of food or medicine for preventing or treating antibiotic-associated diarrhea (CN202210349140.4 pending)

As people's awareness of probiotics gradually increases, the application of probiotic strain raw materials in food, health products or medicines is booming. Wecare Probiotics specializes in probiotic strains, fermented food strains, beneficial microbial flora and In the research, development, production and application of human health, we conduct comprehensive research on high-quality strains of nature, explore probiotics and human health, and provide customers with high-quality probiotic strain raw materials and solutions.