Probiotic Agent Containing Bifidobacterium longum subsp. longum BL21 in Growth and Height Development Support

Abstract

This invention pertains to a probiotic composition designed to promote development and height increase, containing two strains: Bifidobacterium longum subsp. longum BL21 and Bifidobacterium breve BBr16. The combination of these strains exhibits synergistic effects that enhance nutritional absorption, promote bone health, and strengthen the immune system. Key benefits include increased bone density, accelerated skeletal growth, and enhanced gut microbiota diversity and stability.

Claims

1. Probiotic Composition
The composition contains Bifidobacterium longum subsp. longum BL21 (CGMCC No.10452) and Bifidobacterium breve BBr16 (CGMCC No.24471) in a specific ratio. These strains collaborate to enhance growth and height increase.

2. Ratio of Strains
The ratio of Bifidobacterium longum subsp. longum BL21 to Bifidobacterium breve BBr16 ranges from 1:10 to 10:1.

3. Viability
The total viable bacteria count in the composition is not less than 1×10⁸ CFU/mL or 1×10⁸ CFU/g.

4. Formulations
The probiotic composition can be formulated into solutions, lyophilized powders, capsules, tablets, or granules.

5. Preparation of Solution
The preparation involves fermenting both strains separately, centrifuging the fermentation broth, and mixing the bacterial suspensions in specific proportions.

6. Preparation of Lyophilized Powder
Both strains are separately fermented, centrifuged, mixed with protective agents, and lyophilized. The powders are then combined in specific proportions to create the final product.

Technical Field

The invention falls under the technical field of probiotics, specifically a probiotic agent that promotes growth and height increase, designed to enhance skeletal development, immune function, and overall health.

Background

Probiotics have demonstrated potential in promoting infant bone development and enhancing immune function by improving gut nutrient absorption. However, probiotic applications in promoting overall growth are still underdeveloped. This invention addresses this gap by introducing a composition that synergistically combines Bifidobacterium longum subsp. longum BL21 and Bifidobacterium breve BBr16, providing a new strategy to support growth and height development.

Detailed Description

The synergy between Bifidobacterium longum subsp. longum BL21 and Bifidobacterium breve BBr16 significantly enhances their effects on growth and bone health compared to using either strain alone. Key benefits include:

1. Improved Nutrient Absorption and Utilization
The strains help in better absorption of essential nutrients and activate growth factors that promote development.

2. Bone Health and Growth

  
These probiotics significantly increase bone density, enhance bone growth, and accelerate skeletal development.

3. Immune System Modulation
They enhance local gut immunity by increasing immune responses and mucosal defenses, thus improving resistance to pathogens.

4. Gut Microbiota Diversity
The probiotics contribute to the diversity and stability of the gut microbiota, which in turn supports improved gut health and function.

Applications

1. Growth Promotion
The probiotic composition can be used in the preparation of products aimed at promoting the growth and development of the host, particularly in infants and young children.

2. Bone Growth
It can be formulated into supplements or functional foods that support bone metabolism and growth.

3. Immune Enhancement
The probiotic can also be applied in products designed to enhance the immune system's resilience, particularly through gut health.

Experimental Evidence

The invention includes results from animal studies, demonstrating the probiotic composition's efficacy:

· Mouse Study:

The probiotic composition was tested on newborn mice. Results indicated significant growth promotion, with increases in body length, bone density, and bone growth observed, especially in groups treated with the combination of Bifidobacterium longum subsp. longum BL21 and Bifidobacterium breve BBr16.

· Gut Microbiota Analysis:
The composition increased the diversity and richness of gut microbiota, which is associated with improved overall health and disease resistance.

Conclusion

This patent introduces a novel and effective probiotic composition aimed at promoting growth, height increase, and skeletal health, with additional benefits of boosting immune function and gut health. The synergistic effects of Bifidobacterium longum subsp. longum BL21 and Bifidobacterium breve BBr16 make this composition a valuable addition to health supplements, especially for young children and individuals needing bone growth support.