Application of Lacticaseibacillus casei LC16 in Enhancing Bone Health

Introduction

Bone health is a crucial aspect of overall human health, especially as the population ages. Conditions like osteoporosis, characterized by reduced bone mass and deteriorated bone structure, pose significant health risks, particularly to postmenopausal women and the elderly. Recent research has highlighted the role of gut microbiota in bone metabolism, suggesting that probiotics could be beneficial in promoting bone health and preventing osteoporosis.

The Probiotic Strain: Lacticaseibacillus casei LC16

Identification and Preservation Lacticaseibacillus casei LC16 is a novel strain isolated from a dairy product sample in Inner Mongolia. It has been preserved with the identifier CGMCC No.24110, with the deposit date of December 15, 2021.

Key Features and Benefits

• Bone Density Improvement: Lacticaseibacillus casei LC16 significantly increases bone density and promotes calcium absorption.

•  Cellular Activity: Lacticaseibacillus casei LC16 stimulates osteoblast proliferation, inhibits osteoclasts, and regulates bone metabolism.

Applications in Osteoporosis Treatment

Lacticaseibacillus casei LC16 is utilized in products aimed at improving or treating osteoporosis. These products can be dietary supplements or pharmaceuticals.

Formulations

• Probiotic Supplements: Incorporating a live count of at least 1×10^8 CFU/mL or CFU/g.

• Probiotic Blends: Combining Lacticaseibacillus casei LC16 with other strains such as Lacticaseibacillus casei LC86 (CGMCC No.1.12731) and Limosilactobacillus reuteri LR08 (CGMCC No.1.12733) to enhance efficacy.

Additional Components

• Protectants: Soybean powder

• Prebiotics: Inulin, oligosaccharides, resistant starch, spirulina, polydextrose, α-lactalbumin, lactoferrin

Experimental Validation

Isolation and Screening The strain was isolated from traditional dairy products and screened based on its ability to withstand acidic conditions, bile salts, and produce short-chain fatty acids (SCFAs).

Growth and Stability The strain exhibits robust growth and stability under optimized culture conditions, demonstrating high potential for industrial application.

Conclusion

Lacticaseibacillus casei LC16 represents a promising probiotic for enhancing bone health and preventing osteoporosis. Its ability to increase bone density, improve calcium absorption, and regulate bone metabolism makes it a valuable component in dietary supplements and therapeutic products targeting bone health.