A Composite Probiotic for Promoting Intestinal Iron Ion Absorption and its Application

Abstract:

This invention relates to a composite probiotic designed to enhance the absorption of iron ions in the intestines and its various applications. The composite probiotic comprises Lactobacillus gasseri LG08 (CGMCC No. 16131) and Lactobacillus johnsonii LJ09 (CGMCC No. 20123). The combination of these two strains exhibits a synergistic effect in promoting iron absorption, enhancing the bioavailability of iron while maintaining high safety and low resistance.

Keywords:

Probiotic, Iron Absorption, Lactobacillus gasseri, Lactobacillus johnsonii, Intestinal Health, Bioavailability, Nutritional Supplement

Introduction

Iron is an essential nutrient for human health, playing a critical role in oxygen transport and DNA synthesis. However, iron deficiency remains a prevalent issue worldwide, leading to conditions such as anemia, immune dysfunction, and neurological disorders. Traditional iron supplements often have limited efficacy and adverse side effects. Probiotics have emerged as a promising alternative to enhance iron absorption naturally.

Background

The gut microbiota significantly influences iron absorption and overall health. Beneficial bacteria can enhance iron absorption by interacting with the host's metabolic processes and modifying gut permeability. This patent introduces a novel composite probiotic strategy to address iron deficiency through improved iron absorption in the intestines.

Composition  

The composite probiotic consists of two specific strains:  

- Lactobacillus gasseri LG08 (CGMCC No. 16131)

- Lactobacillus johnsonii LJ09 (CGMCC No. 20123)

These strains are combined in ratios ranging from 1:10 to 10:1 to optimize their synergistic effects on iron absorption.

Preparation Method

The preparation involves the following steps:  

1. Activation: Activate LG08 and LJ09 strains.

2. Cultivation: Inoculate the strains in a suitable culture medium.

3. Centrifugation: Centrifuge the culture to obtain the bacterial cells.

4. Suspension: Resuspend the cells in a protective agent solution.

5. Freeze-drying: Freeze-dry the suspension to produce a stable probiotic powder.

Forms of the Microecological Preparation

The probiotic can be formulated into various delivery forms, including:  

- Lyophilized powder

- Capsules

- Tablets

- Granules

Additional Components

The probiotic preparation may include protective agents like skim milk, gelatin, dextrin, and functional additives such as oligosaccharides, inulin, and spirulina to enhance stability and efficacy.

Applications  

The composite probiotic can be used in the following areas:

- Nutritional Supplements: To enhance dietary iron absorption.

- Functional Foods: Incorporated into foods aimed at improving iron intake.

- Medical Treatments: Prevent, alleviate, or treat iron deficiency-related diseases, including iron deficiency anemia, atherosclerosis, neuropathy, and immune dysfunction.

Experimental Results  

Studies involving animal models demonstrated significant improvements in iron absorption with the use of the composite probiotic. Key findings include:

- Increased total iron binding capacity in blood.

- Higher iron concentration in the liver, pancreas, and duodenum.

- Enhanced platelet concentration in blood.

Conclusion

This patent presents a novel and effective approach to improving iron absorption through a composite probiotic. The synergistic action of Lactobacillus gasseri LG08 and Lactobacillus johnsonii LJ09 offers a safe and efficient solution to iron deficiency, with potential applications in both the health food and pharmaceutical industries.

References  

- CGMCC (China General Microbiological Culture Collection Center) Data

- Experimental Data on Probiotic Efficacy and Iron Absorption