A preparation method of lyophilized Lactobacillus acidophilus powder. The technological process includes fermentation and cultivation of Lactobacillus acidophilus, separation of fermentation liquid, emulsification of bacterial sludge and vacuum freeze-drying of emulsified liquid. It is characterized by fermentation and cultivation of strains: Lactobacillus strains are connected to a triangular flask liquid medium for 30-40° Cultivate for 10-30 hours at C; then connect to a large fermenter for fermentation at 35-40°C for 10-30 hours; Sterilize at 115*C for 15 minutes; there are also strains that are fermented and expanded for 10-30 hours, fermentation broth The pH value is 5.5~6.5. 0D600 is above 2.0: Emulsion vacuum freeze-drying: first pre-freeze at -60~-40°C for 1~5 hours, then vacuum freeze-dry at -60°C and 1~8Pa vacuum 10~30 hours. The 1.0x10"cfu/g Lactobacillus vulgaris Donggan bacterial powder prepared by the method of the present invention can be stored at -18*C for 18 months, and its activity and stability are not affected.

The present invention is a method for preparing freeze-dried bifidobacteria powder. The process flow includes fermentation and cultivation of bacteria, separation of fermentation liquid, emulsification of bacteria mud and vacuum freeze drying of emulsified liquid, and is characterized in that the fermentation and cultivation of bacteria are: Bacillus strains are connected to the triangular flask liquid medium and cultured at 30-40°C for 10-30 hours, and sterilized at 115°C for 15 minutes: then connected to the large fermenter and fermented and cultured at 35-40°C for 10-30 hours; Sterilize at 115°C for 15 minutes, the pH of the fermentation broth is 5.5~6.5. 0D600 is above 2.0: Emulsion vacuum freeze-drying: first pre-freeze at -60~-40°C for 1~5 hours, then at -60°C , Vacuum freeze-drying under 1~8Pa vacuum degree for 10~30 hours. The lyophilized bifidobacterium powder prepared by the method of the invention can be stored at -18°C for 18 months, and its activity and stability are not affected.

The present invention discloses a method for improving the ability of lactic acid bacteria to adhere to intestinal epithelial cells, including the following steps: (1) Lactobacillus or Porococcus seed solution cultivation: (2) The seed solution is 3%-4% (V /v) Inoculate the amount of inoculum into a liquid medium containing a certain concentration of salt, and culture it at rest; (3) Centrifuge to obtain bacterial cells. The invention improves the ability of lactic acid bacteria to adhere to intestinal epithelial cells by culturing lactic acid bacteria in a salt-containing medium. The experiment has good repeatability and provides a new method for improving the ability of lactobacilli to adhere to intestinal epithelial cells.